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Induction of rat α 2 ‐macroglobulin in vivo and in hepatocyte primary cultures: synergistic action of glucocorticoids and a Kupffer cell‐derived factor
Author(s) -
Bauer Joachim,
Birmelin Manfred,
Northoff Gerd-Hinrich,
Northemann Wolgang,
Tran-Thi Thuy-Anh,
Ueberberg Heinz,
Decker Karl,
Heinrich Peter C.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80987-4
Subject(s) - hepatocyte , macroglobulin , in vivo , kupffer cell , microbiology and biotechnology , primary (astronomy) , chemistry , action (physics) , in vitro , biology , biochemistry , immunology , physics , quantum mechanics , astronomy
Turpentine injection into rats elicits enhanced secretion of acute phase proteins inducing α 2 ‐macroglobulin (α 2 M). Hypophysectomized rats, however, do not respond in this way unless dexamethasone is given together with turpentine. On the other hand, dexamethasone injection alone did not result in an induction of α 2 M synthesis. When a medium of Kupffer cell cultures was added to hepatocytes, a dose‐dependent stimulation of α 2 M synthesis of up to 4‐fold after 10–12 h was observed. However, the presence of low concentrations (10 −9 M) of dexamethasone was essential for the stimulatory effect. We conclude that the acute phase induction of α 2 M in hepatocytes requires the synergistic action of glucocorticoids and a non‐dialysable factor secreted by Kupffer cells.