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Plasminogen activator activity in differentiating leukemia cells
Author(s) -
Kidron M.,
Nachshon I.,
Mayer M.,
Fibach E.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80982-5
Subject(s) - plasmin , plasminogen activator , chemistry , cell culture , leukemia , tetradecanoylphorbol acetate , microbiology and biotechnology , tissue plasminogen activator , biochemistry , endocrinology , immunology , biology , enzyme , protein kinase c , genetics
Plasminogen activator (PA) activity of human promyelocytic leukemia cell line HL‐60 was assayed by following the conversion of plasminogen to plasmin and the plasmin‐mediated hydrolysis of 14 C‐labeled globin. When HL‐60 cells were induced to differentiate into macrophages by 12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate (TPA), cell‐associated PA activity and secretion of PA into the conditioned medium increased profoundly. PA activity increased earlier and as a result of lower concentrations of TPA than the ability of the cells to adhere. Exposure to 10 −6 M dexamethasone did not prevent TPA‐induced adherence and produced a slight inhibition of cellular PA activity. These findings imply that TPA‐induced differentiation of HL‐60 cells to macrophage‐like cells is associated with induction of PA activity.