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Methylation of a middle repetitive DNA sequence class during differentiation in Friend erythroleukemia cells
Author(s) -
Tolberg Mary Ellen,
Smith Steven S.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80951-5
Subject(s) - biology , dna methylation , methylation , dna , repeated sequence , plasmid , cell division , microbiology and biotechnology , genetics , homologous chromosome , cell , gene , gene expression , genome
We have examined the methylation patterns within middle repetitive sequences in Friend erythroleukemia cells. Mouse‐interspersed‐family‐1 (MIF‐1) and a group characterized by a 1350‐bp Eco‐Bam fragment cloned into pBR322 as plasmid pFS‐13, are both less modified in Friend cell DNA than in normal tissue DNA. The pattern of methylation present in pFS‐13 homologous sequences was found to be stable during cell division, i.e., somatically inherited, and stable during differentiation induced by HMBA.