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Evidence for a two‐step process in prostaglandin secretion
Author(s) -
Thomas Jeannie Marie-France,
Hullin Françoise,
Chap Hugues,
Douste-Blazy Louis
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80941-2
Subject(s) - secretion , chemistry , prostaglandin , process (computing) , biochemistry , computer science , programming language
Cultured endothelial cells (EC) from human umbilical vein were incubated with [U‐ 14 C]arachidonic acid (AA) followed by a challenge with thrombin (2 ) or calcium ionophore A23187 (5 μM) for 0.5–10 min at 37°C. In both cases, AA was rapidly liberated from phospholipids and converted into prostaglandin I 2 (PGI 2 ), as determined by the radioactivity of the stable derivative 6‐keto‐PGF 1α . Maximal liberation of AA and synthesis of PGI 2 were‐achieved within 2 min, but the two compounds first accumulated in EC prior to their release into supernatants. This finding, which was never reported before, raises the question of the mechanism of AA and PG release through the cell membranes and offers a convenient model to investigate this still obscure process.