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31 P‐nuclear magnetic resonance studies of intact plasmodia of Physarum polycephalum
Author(s) -
Kohama Kazuhiro,
Tanokura Masaru,
Yamada Kazuhiro
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80932-1
Subject(s) - physarum polycephalum , phosphocreatine , physarum , mycetozoa , chemistry , intracellular , myosin , resonance (particle physics) , nuclear magnetic resonance , biophysics , actin , biochemistry , biology , physics , dictyostelium , energy metabolism , atomic physics , gene , endocrinology
31 P‐nuclear magnetic resonance spectra were obtained from intact plasmodial cells of Physarum polycephalum , where cytoplasmic streaming is generated by actin‐myosin‐ATP interaction. Several peaks were resolved and identified. They included ATP, ADP, orthophosphate and polyphosphates. Peaks for phosphocreatine, phosphoarginine or AMP were not detected. The intracellular pH and concentrations of ATP and free Mg 2+ were estimated to be pH 6.9, 0.2–0.5 mM, and about 1 mM, respectively.