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Solubilization and reconstitution of the Na + ‐motive NADH oxidase activity from the marine bacterium Vibrio alginolyticus
Author(s) -
Tokuda Hajime
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80925-4
Subject(s) - vibrio alginolyticus , bacteria , solubilization , chemistry , vibrio , marine bacteriophage , vibrionaceae , oxidase test , microbiology and biotechnology , enzyme , biochemistry , biology , genetics , gene
The Na + ‐motive NADH oxidase activity from Vibrio alginolyticus was extracted with octylglucoside and reconstituted into liposomes by dilution. On the addition of NADH, the reconstituted proteoliposomes generated Δψ (inside positive) and ΔpH (inside alkaline) in the presence of a proton conductor CCCP, and accumulated Na + in the presence of valinomycin. These results indicate that the NADH oxidase activity, reconstituted in opposite orientation, leads to the generation of an eletrochemical potential of Na + by the influx of Na + .