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Platelet‐activating factor stimulates phospholipase in quiescent Swiss mouse 3T3 fibroblast
Author(s) -
Kawaguchi Hideaki,
Yasuda Hisakazu
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80918-7
Subject(s) - lysophosphatidylcholine , arachidonic acid , platelet activating factor , diacylglycerol kinase , 3t3 cells , phospholipase , phospholipase a2 , chemistry , phospholipase c , lysophosphatidic acid , biochemistry , platelet , lysophosphatidylethanolamine , lipid signaling , phospholipid , phosphatidylcholine , biology , endocrinology , enzyme , protein kinase c , immunology , transfection , receptor , membrane , gene
Platelet‐activating factor(PAF) induced the rapid hydrolysis of phospholipids in Swiss mouse 3T3 fibroblasts prelabeled with [ 3 H]arachidonic acid or [ 3 H]choline. Up to 23% of the [ 3 H]arachidonic acid incorporated in phospholipids was released into the medium when PAF was added to cells. We also observed increases of diacylglycerol and lysophosphatidylcholine in the medium and cells after the addition of PAF. These results support the possibility that platelet‐activating factor stimulates phospholipase A 2 and C activities in 3T3 cells.