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Antibodies to guanosine
Author(s) -
Iswari S.,
Jacob T.M.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80908-4
Subject(s) - immunogen , guanosine , antibody , chemistry , bovine serum albumin , rna , population , affinity chromatography , biochemistry , periodate , microbiology and biotechnology , dna , biology , monoclonal antibody , enzyme , gene , immunology , medicine , environmental health
Bovine serum albumin conjugates of guanosine prepared by the periodate method was used as immunogen to elicit guanosine antibodies in rabbits. The specificities of the antibodies were studied by the inhibition of their binding to [ 3 H]G ox‐red , [ 32 P]DNA and [ 3 H]RNA by related non‐radioactive compounds. A population of antibodies is specific to G ox‐red with an average association constant of around 10 7 M −1 at 4°C. There are a population of antibodies which bind to [ 32 P]ssDNA and [ 3 H]RNA specifically at guanosine residues. RNA binding antibodies were separated into two populations by affinity chromatography.