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Primary culture, cellular stress and differentiated function
Author(s) -
Wolffe Alan P.,
Tata Jamshed R.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80902-3
Subject(s) - phenotype , function (biology) , biology , microbiology and biotechnology , gene expression , cellular differentiation , cell type , gene , isolation (microbiology) , loss function , cell , genetics , bioinformatics
Isolation of specialized cell types for the analysis of tissue‐specific gene function often results in loss of the differentiated phenotype. Examples of this type of phenotypic change following tissue disaggregation are reviewed together with possible explanations. Close similarities between the effects of cell isolation with those of other cellular stresses such as heat or anoxia point to common biochemical mechanisms being involved. This suggests that the study of freshly isolated cells will contribute significantly to out understanding of the nature of cellular stress and its consequences for the maintenance of phenotype and induction of tissue specific gene expression.