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Yeast 6‐phosphofructo‐2‐kinase
Author(s) -
Yamashoji Shiro,
Hess Benno
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80871-6
Subject(s) - yeast , sephadex , chemistry , chromatography , biochemistry , fructose , sepharose , size exclusion chromatography , enzyme , microbiology and biotechnology , biology
Yeast 6‐phosphofructo‐2‐kinase (PFK2) was purified about 4000‐fold by CM‐Sephadex and Blue Sepharose chromatographies. Yeast contained two kinds of PFK2, both with apparent M r values of 250 000 as estimated by gel filtration. The apparent K m values of the major components for fructose 6‐phosphate (F6P) and ATP were estimated as 0.5 and 0.7 mM, respectively.