Premium
Nucleotide sequence of a yeast tRNA Arg 3A gene and its transcription in a homologous in vitro system
Author(s) -
Villanueva Jorge,
Bull Paulina,
Valenzuela Pablo,
Venegas Alejandro
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80854-6
Subject(s) - gene , yeast , biology , genetics , nucleic acid sequence , transcription (linguistics) , dna , transfer rna , coding region , microbiology and biotechnology , genome , rna , philosophy , linguistics
Twelve bacterial clones containing complementary sequences to yeast tRNA Arg 3 were isolated from a gene library. The size of the yeast Bam HI inserts ranges from 5.4 to 10 MDa. There are at least 6 copies of this gene in different loci of the yeast genome. Insert from clone pYAT‐3 was mapped, and the presence of a tRNA Arg 3A gene was confirmed by DNA sequence. The coding region is colinear with the transcriptional product. Unlike other reported tRAN Arg 3A genes, this one is not linked to a tRNA Asp gene. In vitro transcription using a yeast extract produces a transcript of 76 ± 1 bases.