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Coupling between phosphatidylinositol metabolism and cdc 28 gene product of Saccharomyces cerevisiae
Author(s) -
Dudani Anil Kumar,
Prasad Rajendra
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80851-0
Subject(s) - inositol , phosphatidylinositol , saccharomyces cerevisiae , yeast , gene product , pi , microtubule , chemistry , metabolism , biochemistry , colcemid , gene , microbiology and biotechnology , biology , signal transduction , gene expression , receptor
It was shown that the decrease in phosphatidylinositol (PI) content in cdc 28 G 1 ‐cells was due to a defect in inositol transport. This decrease in inositol transport was linked to microtubular function which was evident by the effect of a microtubular disrupting agent (colcemid) on inositol transport in stationary phase A364A cells. The involvement of PI in yeast G 1 phase was further substantiated by the observation that o ‐phenanthroline, which blocks yeast cells in G 1 phase, could inhibit inositol transport and PI levels as well. It is proposed that the regulation of PI metabolism is mediated by the gene cdc 28 and that microtubules may play a major role in the mechanism of action of this gene product.