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Reconstitution of Azotobacter vinelandii ferredoxin I as a {2[4Fe‐4S] 1+/2+ } protein
Author(s) -
Morgan T.V.,
Stephens P.J.,
Burgess B.K.,
Stout C.D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80848-0
Subject(s) - azotobacter vinelandii , ferredoxin , chemistry , azotobacteraceae , yield (engineering) , azotobacter , metalloprotein , bacteria , biochemistry , physics , organic chemistry , enzyme , biology , nitrogen fixation , nitrogenase , genetics , nitrogen , thermodynamics
As normally isolated, Azotobacter vinelandii ferredoxin I (Fd I) is a {[4Fe‐4S],[3Fe‐3S]} protein: (7Fe)Fd I. We report that anaerobic reconstitution of Fd I from its apoprotein yields a protein whose spectra are distinct from those of (7Fe)Fd I and typical of bacterial ferredoxins. We identify this new form of Fd I as a {2[Fe‐4S]} protein: (8Fe)Fd I. (8Fe)Fd I is unstable in air and decomposes to give a ∼ 10% yield of (7Fe)Fd I. These results increase the probability that (8Fe)Fd I is the form of Fd I occurring in vivo and that (7Fe)Fd I results from oxidative degradation during purification.