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Intermediates in 16‐androstene biosynthesis in neonatal porcine testis
Author(s) -
Kwan T.K.,
Honour J.W.,
Gower D.B.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80841-8
Subject(s) - pregnenolone , derivatization , chromatography , dehydroepiandrosterone , chemistry , gas chromatography , metabolism , capillary gas chromatography , gas chromatography–mass spectrometry , microsome , mass spectrometry , biosynthesis , biochemistry , steroid , hormone , androgen , in vitro , enzyme
The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3‐week old piglets. Metabolites identified after derivatization by capillary gas‐chromatography and further by combined gas chromatography‐mass spectrometry were: 17‐hydroxy‐ and 16α‐hydroxypregnenolone, dehydroepiandrosterone, 5‐androstenediols, 5‐pregnenediol and andien‐β. Pregnenolone and 16‐dehydropregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas‐chromatography.