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Conversion of 1‐aminocyclopropane‐1‐carboxylic acid to ethylene in submitochondrial particles isolated from plants
Author(s) -
Vinkler Chana,
Apelbaum Akiva
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80833-9
Subject(s) - submitochondrial particle , ethylene , 1 aminocyclopropane 1 carboxylic acid , chemistry , propyl gallate , pisum , sativum , nuclear chemistry , organic chemistry , biochemistry , chromatography , enzyme , botany , biology , antioxidant , catalysis
Conversion of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) to ethylene was studied in submitochondrial particles (SMP) which were prepared by sonication of the mitochondrial fraction isolated from 7‐day‐old etiolated pea ( Pisum sativum var. Kelvedon Wonder) seedlings. The reaction was enhanced by the addition of Mn 2+ and had a maximal rate at pH 8.0. Conversion of ACC to ethylene was inhibited under anaerobic conditions and by the addition of KCN, EDTA, NaN 3 , n ‐propyl gallate and CoCl 2 . Addition of the uncouplers 2,4‐DNP, CCCP and FCCP, however, did not inhibit the reaction. Structural analogs of ACC inhibited ACC conversion to enthylene by SMP. The structural analog of methionine, α‐keto‐γ‐methylthiobutyric acid (KMB), was converted to ethylene by SMP at a rate which was only about 2% that of ACC conversion to ethylene.