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Solid‐phase biosynthesis on high performance thin‐layer plates of blood group glycosphingolipids II
Author(s) -
Samuelsson Bo E.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80830-3
Subject(s) - biosynthesis , layer (electronics) , chemistry , thin layer chromatography , amphiphile , phase (matter) , matrix (chemical analysis) , carbohydrate , biochemistry , chromatography , combinatorial chemistry , organic chemistry , polymer , gene , copolymer
A new method for the study of carbohydrate chain biosynthesis of amphipathic glycosphingolipids is presented. The method takes advantage of the thin‐layer plate which is used as a solid matrix of precursor glycosphingolipids in biosynthetic experiments after chromatographic development of the precursor sample. The method is shown to be simple, fast and sensitive. It minimizes the risk of adding unwanted exogenous precursors and abolishes the need for tedious purification of products after incubation. The method opens up new possibilities for the biosynthetic study of mixtures of glycosphingolipids.