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On a non‐pyridine nucleotide‐dependent 2‐oxo‐acid reductase of broad substrate specificity from two Proteus species
Author(s) -
Neumann Stefan,
Simon Helmut
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80826-1
Subject(s) - proteus vulgaris , flavin group , enzyme , proteus mirabilis , chemistry , reductase , substrate (aquarium) , proteus , biochemistry , stereochemistry , pyridine , nucleotide , biology , escherichia coli , organic chemistry , ecology , gene
Proteus mirabilis and Proteus vulgaris contain in the crude extract a non‐pyridine nucleotide dependent reductase for 2‐oxo‐acids as well as 2‐oxo‐dicarboxylic acids of specific activities of 1–10 units/mg protein. The enzyme catalyzes the reduction of very different 2‐oxo‐acids to the corresponding (2R)‐hydroxy‐acid. Reduced methyl‐ or benzylviologen act as artifical electron donors. The membrane‐bound enzyme has been enriched 167‐fold to a purity 90% as judged by electrophoresis. It seems that the enzyme does not contain flavin.