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An electrogenic proton pump in plasma membranes from the cellular slime mould Dictyostelium discoideum
Author(s) -
Strandmann Ralph Pogge-von,
Kay Robert R.,
Dufour Jean-Pierre
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80781-4
Subject(s) - dictyostelium discoideum , membrane , diaphragm pump , slime mold , chemistry , mycetozoa , dictyostelium , proton pump , biochemistry , biophysics , microbiology and biotechnology , atpase , biology , enzyme , materials science , nanotechnology , micropump , gene
Plants and fungi possess an outwardly directed plasma membrane proton pump that may regulate intracellular pH. We provide the first demonstration that amoebae of the slime mould Dictyostelium discoideum also possess a similar proton pump. It can be assayed either as an ATPase activity in highly purified plasma membranes or as a proton pump, after solubilization and reconstruction into liposomes. The pump is inhibited by vanadate, diethylstilbestrol (DES) and miconazole but not by azide or ouabain. The proton pump described here may represent the target for the action of DES and miconazole, both of which have previously been shown to induce stalk cell formation during the in vitro development of Dictyostelium .