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NAD + kinase: molecular weight determination by low‐angle laser‐light scattering
Author(s) -
Gabriel Mina K.,
McGuinness Eugene T.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80780-2
Subject(s) - dimer , monomer , chemistry , virial coefficient , mole , enzyme , analytical chemistry (journal) , biochemistry , chromatography , physics , organic chemistry , thermodynamics , polymer
Low‐angle laser‐light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD + kinase (NADK). The weight‐average molecular weight ( w ,) was found to be 275000 ± 15000. The corresponding value for the second virial coefficient was −1.65 × 10 −3 ml·mol·g 2 . The value for M w is in close accord with estimates reported for pigeon liver (270000) and C. utilis (260000) NADK. If the active enzyme is a dimer, the weight difference between pigeon/chicken liver and rabbit liver (136000) NADK would indicate that the latter enzyme is an active monomer unit.