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The primary structure of the gene encoding yeast ribosomal protein L 16
Author(s) -
Leer Robert J.,
van Raamsdonk-Duin Mary M.C.,
Mager Willem H.,
Planta Rudi J.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80771-1
Subject(s) - ribosomal protein , gene , genetics , biology , ribosomal rna , microbiology and biotechnology , primer extension , primer (cosmetics) , messenger rna , chemistry , ribosome , rna , organic chemistry
As part of our studies on the molecular basis for the coordinate expression of ribosomal protein genes in yeast we analyzed the primary structure of the gene encoding protein L16 of the large ribosomal subunit including the flanking sequences. L 16 turned out to be a ribosomal protein with a molecular mass of 22662 Da and a net charge of +12. Both the 5'‐ and the 3'‐end of the L16 mRNA were mapped by primer extension and S 1 nuclease analysis. In the DNA regions flanking the coding sequence several conserved elements are present that may be involved in transcription initiation or termination.