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Measurement of the rotational behaviour of virus particles during adsorption to the host cell surface
Author(s) -
Bayer M.E.,
Morrison I.,
Cherry R.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80762-0
Subject(s) - rotational diffusion , virus , capsid , adsorption , chemistry , vesicle , biophysics , diffusion , membrane , analytical chemistry (journal) , virology , chromatography , biology , biochemistry , physics , molecule , organic chemistry , thermodynamics
The rotational diffusion of bacteriophage ϵ15 was measured before and after virus adsorption to outermembrane vesicles of the host Salmonella anatum . The virus capsid was labeled with eosin isothiocyanate, and the decay of transient dichroism following dye excitation by pulses of plane‐polarized light was measured. From the data, the rotational diffusion constant of the unadsorbed virion and its hydrodynamic diameter were estimated and found to be consistent with electron microscopic measurements of the capsid dimensions. Addition of outer membrane vesicles of S. anatum to the virus suspension revealed the immobilization of the virus particles on the membrane surface.