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H1 histone, polylysine and spermine facilitate nucleosome assembly in vitro
Author(s) -
Bogdanova E.S.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80760-7
Subject(s) - polylysine , nucleosome , histone , spermine , dna supercoil , histone octamer , histone h1 , dna , biophysics , linker dna , chemistry , in vitro , chromatosome , microbiology and biotechnology , biochemistry , biology , dna replication , enzyme
Nucleosome formation has been studied in a system containing relaxed Col El DNA, core histones and an extract of Drosophila embryos. The formation of nucleosomes was established by the introduction of supercoils into DNA. The degree of DNA supercoiling was shown to be higher if nucleosomes were assem‐bled in the presence of the H1 histone, polylysine ( M r 20000) or spermine. These agents do not stimulate relaxation and are the more effective the earlier they are added to the reaction. Thus, the H1 histone, polylysine and spermine facilitate nucleosome assembly in vitro.