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A protein molecule as a bioanalytical device
Author(s) -
Morozov Victor N.,
Morozova Tamara Ya.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80755-3
Subject(s) - chemistry , papain , bioanalysis , molecule , biophysics , combinatorial chemistry , enzyme , biochemistry , chromatography , organic chemistry , biology
Cross‐linked protein solids are proposed as new types of chemomechanical sensors for identification of biological molecules and measurement of their concentration. New sensors make use of the ability of proteins to specifically bind its ligands and to alter their conformation upon the binding. The use of immobilized proteins enables these conformational changes to be detected through the changes in mechanical properties of protein solid samples (stress, stain or the Young's modulus). Some advantages of the new sensors over enzyme probes are illustrated on considering the properties of papain sensor for α‐ N ‐benzoyl‐L‐arginine ethyl ester.