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Mutations in the regulatory subunit of soluble phosphofructokinase from yeast
Author(s) -
Nadkarni Medha,
Parmar Latika,
Lobo Zita,
Maitra P.K.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80754-1
Subject(s) - phosphofructokinase , yeast , mutant , protein subunit , gene , biochemistry , enzyme , allele , chemistry , biology , genetics , glycolysis
Mutant alleles of the gene PFK2 have been obtained that alter the sensitivity to ATP inhibition of the soluble yeast phosphofructokinase. One of the alleles makes the enzyme sensitive to micromolar concentrations of ATP. Intragenic revertants of PFK2 mutants confirm that the PFK2 gene determines not only the regulatory properties of the soluble enzyme but also the catalytic activity of particulate phosphofructokinase.