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Transformation of leukotriene A 4 methyl ester to leukotriene C 4 monomethyl ester by cytosolic rat glutathione transferases
Author(s) -
Mannervik Bengt,
Jensson Helgi,
Ålin Per,
Örning Lars,
Hammarström Sven
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80753-x
Subject(s) - glutathione , cytosol , chemistry , leukotriene c4 , glutathione transferase , enzyme , leukotriene , transferase , transformation (genetics) , biochemistry , glutathione s transferase , stereochemistry , biology , asthma , gene , immunology
Six major basic cytosolic glutathione transferases from rat liver catalyzed the conversion of leukotriene A 4 methyl ester to the corresponding leukotriene C 4 monomethyl ester. Glutathione transferasc 4‐4, the most active among these enzymes, had a V max of 615 nmol · min −1 · mg protein −1 at 30°C in the presence of 5 mM glutathione. It was followed in efficiency by transferase 3–4 which had a V max of 160 nmol · min −1 · mg −1 under the same conditions. Transferases 1‐1, 1‐2, 2‐2 and 3‐3 had at least 30 times lower V max values than transferase 4‐4.