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Do monoclonal antibodies Tü15 and Tü67 detect heterogeneity of human transferrin receptor molecules?
Author(s) -
Uchańska-Ziegler Barbara,
Wernet Peter,
Liangru Shi,
Ziegler Andreas
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80751-6
Subject(s) - monoclonal antibody , transferrin , transferrin receptor , receptor , microbiology and biotechnology , antibody , monoclonal , biology , population , chemistry , biochemistry , immunology , medicine , environmental health
The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies. Tü 15 and Tü67. Both reagents precipitated from lysates of 125 I‐labeled HL‐60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins detected by monoclonal antibody OKT9. Although sequential immunoprecipitations appeared to demonstrate molecular heterogeneity of transferrin receptors, since the Tü15‐reactive species were fully included in the Tü67‐positive population, but not vice versa, the possible association of Tü15‐reactive molecules with transferrin receptor is also discussed.