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Glycosidic bond rearrangements in isomeric xylobioses by yeast xylan‐degrading enzymes
Author(s) -
Biely Peter,
Petráková Eva
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80626-2
Subject(s) - xylobiose , trisaccharide , chemistry , beta (programming language) , yeast , enzyme , biochemistry , xylose , stereochemistry , xylanase , glycoside hydrolase , fermentation , computer science , programming language
The cells of Cryptococcus albidus induced for xylan‐degrading enzymes are capable of transforming 1,2‐β‐xylobiose and 1,3‐β‐xylobiose into 1,4‐β‐xylobiose, the natural inducer. The conversion involves transglyco‐sylation and hydrolysis catalyzed by β‐xylosidase and β‐xylanase. A probable intermediate of the conversion of 1,2‐β‐xylobiose was isolated and identified as a trisaccharide, 4‐ O ‐β‐xylopyranosyl‐2‐ O ‐β‐xylopyranosyl‐D‐xylopyranose. The trisaccharide is cleaved by purified endo‐1,4‐β‐xylanase of C. albidus mainly at the 1,2‐β‐linkage yielding xylose and 1,4‐β‐xylobiose.