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Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells
Author(s) -
Tomida Mikio,
Yamamoto-Yamaguchi Yuri,
Hozumi Motoo
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80619-5
Subject(s) - antiserum , ehrlich ascites , microbiology and biotechnology , myeloid , polyacrylamide gel electrophoresis , biology , chemistry , neoplasm , biochemistry , tumor cells , antigen , cancer research , immunology , enzyme , genetics
A factor inducing differentiation of mouse myeloid leukemic cells (M1) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was halt‐maximally active at 2 × 10 −11 M. The factor was analyzed by radioiodination, SDS‐polyacrylamide gel electrophoresis and autoradiography. Its M r was 40000–50000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo‐β‐ N ‐acetylglucosaminidase F, but not endo‐β‐ N ‐acetylglucosaminidase H, gave rise to a molecule of M r 20000–28000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of M r 50000–70000 from mouse fibroblast L929 cells.