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Inhibition of glutathione efflux in the recirculating rat liver perfusion by cysteine but not by oxothiazolidine carboxylate, an intracellular cysteine precursor
Author(s) -
Glazenburg Eitjo J.,
Bruggink Jan E.,
Wolters-Keulemans Katja,
Mulder Gerard J.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80614-6
Subject(s) - glutathione , cysteine , cystine , efflux , perfusion , cysteine metabolism , biochemistry , intracellular , chemistry , medicine , enzyme
In the recirculating rat liver perfusion a continuous release of glutathione into the perfusion medium is observed. Addition of L‐cysteine to the perfusion medium immediately arrested this glutathione efflex. The cysteine precursor oxothiazolidine carboxylate did not block the glutathione efflux in spite of the fact that it generated more L‐cysteine inside the liver cells than L‐cysteine itself; L‐cysteine is rapidly oxidized to cystine, that is no longer taken up by the liver. The results suggest that the inhibition of glutathione efflux results from the presence of cystine in the perfusion medium.