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Barbiturate and picrotoxin‐sensitive chloride efflux in rat cerebral cortical synaptoneurosomes
Author(s) -
Schwartz Rochelle D.,
Skolnick Phil,
Hollingsworth Elizabeth B.,
Paul Steven M.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80597-9
Subject(s) - picrotoxin , barbiturate , chemistry , efflux , barbital , pentobarbital , hexobarbital , pharmacology , chromatography , biochemistry , gabaa receptor , receptor , medicine , microsome , enzyme
The effects of various barbiturates and picrotoxin in modifying the efflux of chloride ( 36 Cl − ) was studied in a novel subcellular preparation from rat cerebral cortex, the ‘synaptoneurosome’. Dilution of synaptoneurosomes pre‐loaded with 36 Cl − resulted in rapid efflux of 36 Cl − that could be measured as early as 10 s following dilution. In the presence of barbiturates such as pentobarbital and hexobarbital there was a significant increase in 36 Cl − efflux which was not observed with the pharmacologically‐inactive barbiturate, barbital. The effect of barbiturates in enhancing 36 Cl − efflux was also stereospecific [(−)‐DMBB > (+)‐DMBB] and reversed by picrotoxin. By contrast, picrotoxin alone significantly inhibited 36 Cl − efflux. These data demonstrate pharmacologically relevant Cl − transport for the first time in a subcellular brain preparation.