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Human apolipoprotein A‐II: complete nucleic acid sequence of preproapoA‐II
Author(s) -
Lackner Karl J.,
Law Simon W.,
Brewer H.Bryan
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80590-6
Subject(s) - complementary dna , primer (cosmetics) , microbiology and biotechnology , peptide sequence , biology , cdna library , oligonucleotide , amino acid , nucleic acid , primer extension , nucleic acid sequence , biochemistry , sequence analysis , dna , chemistry , messenger rna , gene , organic chemistry
The complete cDNA nucleic acid sequence of preproapolipoprotein (apo) A‐II, a major protein constituent of high density lipoproteins, has been determined on clones from a human liver ds‐cDNA library. Clones containing ds‐cDNA for apoA‐II were identified in the human liver ds‐cDNA library using synthetic oligonucleotides as probes. Of 3200 clones screened, 4 reacted with the oligonucleotide probes. The DNA sequence coding for amino acids −17 to +17 of apoA‐II were determined by Maxam‐Gilbert sequence analysis of restriction fragments isolated from one of these clones, pMDB2049. The remainder of the cDNA sequence was established by sequence analysis of a primer extension product synthesized utilizing a restriction fragment near the 5'‐end of clone pMDB2049 as primer with total liver mRNA. The apoA‐II mRNA encodes for a 100 amino acid protein, preproapoA‐II that has an 18 amino acid prepeptide and a 5 amino acid propeptide terminating with a basic dipeptide (Arg‐Arg) at the cleavage site to mature apoA‐II.