Premium
Histamine release by pharmacological agents in the absence of external free Ca 2+
Author(s) -
Pintado E.,
Carrasco M.,
Goberna R.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80588-8
Subject(s) - histamine , extracellular , secretion , chemistry , mast cell , biophysics , liberation , potassium , incubation , calcium , endocrinology , biochemistry , medicine , biology , immunology , in vitro , organic chemistry
The involvement of extracellular free Ca 2+ in histamine release was investigated in rat peritoneal mast cells. Incubation of non‐antigenized cells in a media with high extracellular potassium did not increase histamine release. Secretion induced by A23187 and compound in the presence of Ca 2+ requires metabolic energy. In the absence of external free Ca 2+ (2.5 μM) histamine release induced by A23187 is reduced but not abolished. Secretion induced by compound is independent of extracellular Ca 2+ . These results lead us to suggest that mast cell plasma membranes probably lack voltage‐gated Ca 2+ channels and that external Ca 2+ may not be an absolute requisite for histamine secretion.