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Oocyte adenylyl cyclase contains N i , yet the guanine nucleotide‐dependent inhibition by progesterone is not sensitive to pertussis toxin
Author(s) -
Birnbaumer Lutz,
Olate Juan,
Allende Catherine C.,
Allende Jorge E.,
Sekura Ronald D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80562-1
Subject(s) - adenylyl cyclase , pertussis toxin , gs alpha subunit , cholera toxin , biology , adp ribosylation , adcy9 , biochemistry , cyclase , g protein , microbiology and biotechnology , receptor , endocrinology , enzyme , nad+ kinase
The possible susceptibility to pertussis toxin of the guanine nucleotide‐dependent inhibition of oocyte adenylyl cyclase by progesterone was investigated. This action of progesterone is mediated by a membrane bound receptor as opposed to a receptor of cytosolic or nuclear localization. However, the inhibitory effect of progesterone was unaffected by pertussis toxin, even though the oocyte membrane N i was fully ADP‐ribosylated with pertussis toxin, as revealed by lack of further [ 32 P]ADP‐ribosylation on subsequent re‐incubation with pertussis toxin. These results indicate that the action of progesterone, in spite of being nucleotide‐dependent, is either not mediated by N i , suggesting the existence of an additional nucleotide regulatory component, or if mediated by N i , involves a mode of regulation of this coupling protein that is different from that by which all other inhibitory hormones act on adenylyl cyclase.