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Topographical relationships of polypeptides in the photosynthetic membrane of Rhodopseudomonas viridis investigated by reversible chemical cross‐linking
Author(s) -
Peters Jürgen,
Welte Wolfram,
Drews Gerhart
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80501-3
Subject(s) - random hexamer , photosynthetic reaction centre , protein subunit , rhodopseudomonas , chemistry , pigment , rhodospirillaceae , photosynthesis , membrane , stereochemistry , biochemistry , photochemistry , organic chemistry , gene
The B1020 antenna pigment—protein complex of Rhodopseudomonas viridis was isolated and found to contain two polypeptide subunits of apparent M r 8000 and 12000. Near‐neighbour relationships between both antenna 8‐kDa and 12‐kDa polypeptides were revealed by reversible chemical cross‐linking. Cross‐linked homooligomers, probably up to the respective hexamer, were observed. Subunit M of the photochemical reaction center (RC) and the membrane‐bound cytochrome c ‐553/558 were cross‐linked in high yield at a 1:1 stoichiometry. The heavy subunit H of the RC was cross‐linked to the 8‐kDa polypeptide.