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The gene coding for lipoprotein signal peptidase ( lspA ) and that for isoleucyl‐tRNA synthetase ( ileS ) constitute a cotranscriptional unit in Escherichia coli
Author(s) -
Yamada Hisami,
Kitagawa Masanari,
Kawakami Makoto,
Mizushima Shoji
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80496-2
Subject(s) - biology , gene , escherichia coli , coding region , genetics , microbiology and biotechnology , gene expression
The lspA gene coding for lipoprotein signal peptidase is located very close to the ileS gene coding for isoleucyl‐tRNA synthetase on the Escherichia coli chromosome. Deletions were generated in vitro from both ends of the 4.3 kb fragment that carries the lspA gene and the ileS gene, and the expression of the two genes was examined before and after insertion of the trp promoter‐operator at one end. The results indicate that the lspA and ileS genes constitute a cotranscriptional unit in the order of promoter‐ ileS ‐ lspA . The gene order of dnaJ ‐ rpsT ‐ ileS ‐ lspA ‐ dapB around 0.5 min on the E. coli chromosome map was also determined.