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Role of intestinal brush border membrane aminopeptidase N in dipeptide transport
Author(s) -
Antonov V.K.,
Vorotyntseva T.I.,
Bessmertnaya L.Ya.,
Mikhailova A.G.,
Zilberman M.I.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80493-7
Subject(s) - brush border , dipeptide , chemistry , brush , aminopeptidase , membrane , biochemistry , peptide , amino acid , leucine , vesicle , materials science , composite material
The kinetics of uptake of radioactive label from [U‐ 14 C]Gly, L‐[4,5‐ 3 H]Leu and the dipeptide [ 14 C]Gly—L‐[4,5‐ 3 H]Leu by the brush border membrane vesicles of porcine small intestine have been studied. The effect of aminopeptidase N inhibitors and leucine‐binding protein on accumulation rates has also been tested. Comparison of the kinetic parameters for uptake and hydrolysis of Gly—L‐Leu makes it possible to conclude that the dipeptide transfer includes two conjugated steps, viz., hydrolysis catalysed by aminopeptidase N and transport of the resultant free amino acids by a specific carrier.