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The complete nucleotide sequence of the xylanase gene ( xynA ) of Bacillus pumilus
Author(s) -
Fukusaki Eiichiro,
Panbangred Watanalai,
Shinmyo Atsuhiko,
Okada Hirosuke
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80487-1
Subject(s) - xylanase , bacillus pumilus , ribosomal binding site , nucleic acid sequence , biochemistry , peptide sequence , gene , amino acid , start codon , bacillus subtilis , open reading frame , biology , chemistry , nucleotide , ribosome , genetics , enzyme , bacteria , rna
The complete nucleotide sequence of the xylanase (EC 3.2.1.8) gene ( xynA and its flanking regions of Bacillus pumilus IPO, a hyperproducer of xylanase, was determined. A 684 bp open reading frame for xylanase gene was observed. The amino acid sequence of the N‐terminal region of xylanase was determined to be Arg‐Thr‐Ile‐Thr‐, suggesting the processing at Ala 27 of pre‐xylanase. The amino acid composition and M r (22384) of xylanase deduced from DNA sequence agreed with the results obtained with the purified enzyme. The signal sequence consisted of 27 amino acids, of which 3 were basic amino acid residues in the region near the N‐terminus and 15 were hydrophobic amino acid residues. The ribosome binding sequence complementary to the 3′ ‐end of 16 S rRNA of B , subtilis was found 7 bp upstream of the initiation codon, ATG.