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Effect of Mn 2+ and Ca 2+ on O 2 evolution and on the variable fluorescence yield associated with Photosystem II in preparations of Anacystis nidulans
Author(s) -
Pistorius Elfriede K.,
Schmid Georg H.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80482-2
Subject(s) - fluorescence , photosystem ii , chemistry , yield (engineering) , oxygen evolution , quantum yield , chlorophyll fluorescence , photosystem i , manganese , photosynthesis , acceptor , analytical chemistry (journal) , p700 , photochemistry , chromatography , electrochemistry , biochemistry , materials science , electrode , physics , optics , organic chemistry , metallurgy , condensed matter physics
Extraction with EDTA of lyophilized and lysozyme treated preparations of the blue‐green algae Anacystis nidulans resulted in loss of the capacity for photoevolution of O 2 . Reactivation was achieved by the addition of both cations: Mn 2+ and Ca 2+ (or to a smaller extent by Mn 2+ and Sr 2+ ). The dual requirement for Mn 2+ and Ca 2+ could be demonstrated when the O 2 evolution under short saturating light flashes and the variable chlorophyll fluorescence associated with the reduction of the primary acceptor of Photosystem II was examined. The fluorescence experiments in addition showed that incorporation of the cations was a light dependent step, since the fluorescence rise only started after a lag period.