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31 P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes
Author(s) -
Roux Michel,
Champeil Philippe
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80481-0
Subject(s) - solubilization , phospholipid , micelle , membrane , chemistry , vesicle , endoplasmic reticulum , chromatography , bilayer , lipid bilayer , biochemistry , aqueous solution , organic chemistry
Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31 P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the narrow resonance signal characterizing phospolipids inserted into a mixed micelle. Up to 0.25 g detergent/g protein could be incorporated into the membrane without solubilization. Higher detergent concentrations of up to 1.5–2 g detergent/g protein led to gradual solubilization. Although the method allows us to monitor the extent of solubilization of individual phospholipid classes, there was no evidence of either preferential solubilization or retention of a specific class of phospholipids.