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Changes in intracellular pH and inorganic phosphate concentration during and after muscle contraction as studied by time‐resolved 31 P‐NMR
Author(s) -
Tanokura Masaru,
Kazuhiro Yamada
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80480-9
Subject(s) - inorganic phosphate , phosphate , intracellular ph , chemistry , contraction (grammar) , intracellular , muscle contraction , biophysics , biochemistry , medicine , biology
The changes in intracellular pH and concentration of inorganic phosphate (P i ) were studied during and after contraction of bullfrog sartorius muscles by using 31 P‐NMR. The intracellular pH was 7.04 in resting muscles at 4°C and an alkalinization by as much as 0.08 pH unit occurred following 5 s tetanic contraction. During the recovery period after contraction the increased level of intracellular pH returned to the resting level, the time course of which coincided with that of the recovery of the P i concentration. Based on the changes in the intracellular pH and P i concentration, the buffering power as estimated to be 18 mM/pH unit.