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In vivo and in vitro catabolism of native and biologically modified LDL
Author(s) -
Nagelkerke J.Fred,
Havekes Louis,
van Hinsbergh Victor W.M.,
van Berkel Theo J.C.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80477-9
Subject(s) - catabolism , in vivo , umbilical vein , clearance , in vitro , low density lipoprotein , chemistry , incubation , ldl receptor , endothelial stem cell , receptor , lipoprotein , endocrinology , medicine , pharmacology , biochemistry , biology , cholesterol , metabolism , microbiology and biotechnology , urology
Incubation of human low density lipoprotein (LDL) at 37°C in the presence of human umbilical‐vein endothelial cells (EC) causes a time‐dependent shift in the charge and density of LDL. After intravenous injection into rats, native LDL is merely cleared from the circulation by Kupffer cells while EC‐modified LDL is rapidly cleared by endothelial liver cells. The uptake of native LDL by Kupffer cells and EC‐modified LDL by endothelial cells in vivo can be explained by the presence of two different specific receptors on these cell types. It is concluded that the liver endothelial cells form an important protection against a possible atherogenic action of EC‐modified LDL.