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Molecular mass analysis of murine immunosuppressive immunoglobulin G‐binding factors (IgG‐BFs) produced by T‐cell hybrids
Author(s) -
Néauport-Sautès Catherine,
Gelabert Maria-José,
Moncuit Janine,
Fridman Wolf Herman
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80456-1
Subject(s) - antibody , microbiology and biotechnology , immunoglobulin g , chemistry , biology , immunology
Induced and constitutive murine IgG‐binding factors (IgG‐BFs) have been purified by affinity chromatography from supernatants of T‐cells preincubated with or without murine monoclonal IgG1 and IgG2b, respectively. IgG‐BF M r values have been studied by SDS‐polyacrylamide gel electrophoresis (PAGE) after treatment with SDS under conditions which do not noticeably alter their immunosuppressive activities on the secondary in vitro IgG antibody response. Suppression was recovered at M r values of 80000, 40000 and 20000. When induced IgG‐BF was tested, the isotype‐specific suppressive activity was found only at 40 kDa. The 20‐kDa moiety appeared to derive from the 40‐kDa component and the material found at 80 kDa exerted non‐specific immunosuppressive effects. We conclude therefore that isotype‐specific IgG‐BF has an apparent M r of 40000.