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Two protons are pumped from the mitochondrial matrix per electron transferred between NADH and ubiquinone
Author(s) -
Wikström Mårten
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80338-5
Subject(s) - ferricyanide , ubiquinol , mitochondrial matrix , chemistry , mitochondrion , electron transport chain , cytochrome c , proton , cytochrome , photochemistry , coenzyme q – cytochrome c reductase , biochemistry , cytosol , enzyme , physics , quantum mechanics
The spectrophotometric indicators neutral red and safranine were used to determine the relative H + /e − ratios of proton uptake from the mitochondrial matrix, and the q + /e − ratios of electrical charge translocation, during oxidation of β‐OH‐butyrate and succinate by ferricyanide in rat liver mitochondria. With β‐OH‐butyrate both ratios were higher than with succinate by a factor close to 3.0. Since there is full agreement that H + /e − of proton uptake and q + /e − of charge translocation are both equal to unity for oxidation of succinate (or ubiquinol) by ferricytochrome c , the corresponding ratios for oxidation of NADH by ubiquinone and cytochrome c are near 2.0 and 3.0, respectively.