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Detection of two DCCD‐binding components in the envelope membrane of H. halobium
Author(s) -
Konishi Tetsuya,
Murakami Naoyuki
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80334-8
Subject(s) - chemistry , membrane , molecular mass , envelope (radar) , binding site , membrane protein , chromatography , biophysics , biochemistry , biology , enzyme , telecommunications , radar , computer science
From labeling studies using [ 14 C]DCCD, the presence of two distinct DCCD‐binding sites was proved in halobacterial membrane, whose binding constants are 0.03 and 0.08 nmol DCCD/mg envelope protein, respectively. HPLC and SDS—PAGE analysis revealed that the radioactivity bound to these sites migrated with apparent molecular masses of 45 and 10.2 kDa protein components, respectively.