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Separation of endo‐ and exo‐type cellulases using a new affinity chromatography method
Author(s) -
van Tilbeurgh Herman,
Bhikhabhai Ramagauri,
Göran Pettersson L.,
Claeyssens Marc
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80321-x
Subject(s) - cellulase , trichoderma reesei , cellobiose , chemistry , elution , adsorption , chromatography , cellulose , biochemistry , organic chemistry
A new efficient biospecific adsorbent for the purification of cellulases was prepared from p ‐aminobenzyl 1‐thio‐β‐D‐cellobioside and Affigel‐10 (Biorad). The two exocellobiohydrolases, CBH I and CBH II, from Trichoderma reesei QM 9414 were adsorbed, whereas the endoglucanase activities were unretarded. The adsorbed CBH I can be displaced selectively by elution with lactose, whereas cellobiose elutes the CBH II.