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Purification and characterization of ubiquitin from mammalian testis
Author(s) -
Loir M.,
Caraty A.,
Lanneau M.,
Menezo Y.,
Muh J.P.,
Sautiere P.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80318-x
Subject(s) - ubiquitin , chemistry , microbiology and biotechnology , biochemistry , biology , gene
Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH 2 ‐terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.