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Two‐dimensional gel electrophoresis of proteins released from sea urchin nuclei by limited digestion with DNase I
Author(s) -
Takahashi Kazuhide
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80311-7
Subject(s) - blastula , mesenchyme , sea urchin , chemistry , digestion (alchemy) , zygote , gel electrophoresis , microbiology and biotechnology , biochemistry , polyacrylamide gel electrophoresis , biology , embryonic stem cell , embryo , embryogenesis , enzyme , chromatography , gene , gastrulation
The morula and the mesenchyme blastula nuclei contained approx. 30 nuclear proteins which were preferentially released by limited digestion with DNase I, but no proteins were released from sperm nuclei. While most of the proteins released by DNase I digestion were common to the two embryonic stages, 2 and 6 proteins were specific or enriched in morulae and mesenchyme blastulae, respectively.