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The human carcinoma cell line A431 possesses large numbers of functional β‐adrenergic receptors
Author(s) -
Delavier-Klutchko C.,
Hoebeke J.,
Strosberg A.D.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80308-7
Subject(s) - a431 cells , receptor , dihydroalprenolol , adenylate kinase , cyclase , adrenergic receptor , alpha 1b adrenergic receptor , biology , alpha 1d adrenergic receptor , biochemistry , cell surface receptor , cell , microbiology and biotechnology , chemistry , antagonist , cell cycle , beta 3 adrenergic receptor , partial agonist , molecular medicine
The existence of β‐adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1‐[ 3 H]dihydroalprenolol and the hydrophilic antagonist [ 3 H]CGP‐12,177 as β‐adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the β 2 ‐subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human β‐adrenergic receptors.