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Nucleotide substitutions in a yeast mitochondria cis ‐acting mutant located in the last nitron of the apocytochrome b gene
Author(s) -
Bonjardim C.A.,
Nóbrega F.G.
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80292-6
Subject(s) - mutant , gene , nucleotide , genetics , mitochondrial dna , intron , nucleic acid sequence , saccharomyces cerevisiae , biology , yeast , mutation , mitochondrion , dna , microbiology and biotechnology
The region of mitochondrial DNA corresponding to the intron mutant M6‐200 in Saccharomyces cerevisiae D273‐10B has been isolated, and the nucleotide sequence of a 519 by Rsa I fragment has been determined. Three nucleotide substitutions were found at nucleotides + 2650 (G → T), + 2668 (G → A) and + 2798 (A → G), all within the genetically defined location in the gene. Particular significance can be attributed to the first two changes (+ 2650 and + 2668), that can be genetically isolated from the third substitution and, in addition, alter conserved sequence features detected in a study [(1982) Biochimie 64, 867–881] of fungal mitochondrial introns.