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Chemical characterization of cyanogen bromide fragments from the β‐chain of human complement factor C3
Author(s) -
Lundwall Åke,
Hellman Ulf,
Eggertsen Gösta,
Sjöquist John
Publication year - 1984
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(84)80289-6
Subject(s) - cyanogen bromide , chemistry , protein primary structure , peptide sequence , amino acid , polypeptide chain , size exclusion chromatography , sequence (biology) , stereochemistry , biochemistry , enzyme , gene
The isolated β‐chain of human complement factor C3 (C3β) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high‐pressure liquid chromatogaphy, and characterized with respect to their M r , amino acid composition and N‐terminal amino acid sequence. Approx. 30% of the primary structure of C3β was determined. Alignment of the 3 N‐terminal fragments allowed determination of 61 of the amino terminal residues of C3β. This region demonstrated 40% homology with the sequence in the N‐terminal segment of the α‐chain of the cobra venom factor.